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Image Search Results
Journal: bioRxiv
Article Title: New realm of precision multiplexing enabled by massively-parallel single molecule UltraPCR
doi: 10.1101/2023.10.09.561546
Figure Lengend Snippet: (A) Schematic of how optical signature is captured. In this specific example, every partition is scanned serially with 10 different channel settings configured for dye classes I, II, and III. Channel settings include distinct excitation wavelength, indicated by colored vertical lines, emission wavelength, indicated by colored rectangles, and photobleaching events. Data generated in each channel is used to assemble an optical signature for each partition composed of fluorescence intensity values, which unambiguously identifies the target captured in 3D partition positions. Sample of data is shown in the table (B) light sheet images with photobleachable (FAM, Bodipy TMR-X, and Cy5) and photostable (Alexa488, HEX, and Atto647N) dyes before and after a brief photobleaching event. Zoomed in micrographs of individual partitions are shown in the corner of images as examples. (C) UMAP visualization of optical signatures for all positive partitions detected by the UltraPCR Imager analysis pipeline followed by clustering using DBScan . (D) Aggregate optical signature for each cluster (vertical-axis) with circle size indicating normalized intensity value for each channel (horizontal-axis). (E) Number of molecules identified per cluster. This experiment was performed as a duplicate to generate an error bar signifying standard error.
Article Snippet: Multivariable analysis was performed in
Techniques: Generated, Fluorescence